Plasmid_Backbone
pET9d-SYB6

Part:BBa_K3653014:Design

Designed by: Shanny Ackerman, Ella Samuel   Group: iGEM20_Technion-Israel   (2020-10-12)


pET9D with Sybody 68


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 655
    Illegal XbaI site found at 951
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 655
    Illegal NheI site found at 229
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 655
    Illegal BglII site found at 1009
    Illegal BamHI site found at 510
    Illegal XhoI site found at 3915
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 655
    Illegal XbaI site found at 951
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 655
    Illegal XbaI site found at 951
    Illegal NgoMIV site found at 1041
    Illegal NgoMIV site found at 1409
    Illegal NgoMIV site found at 1569
    Illegal NgoMIV site found at 1923
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

No


Source

The Vector: https://www.snapgene.com/resources/plasmid-files/?set=pet_and_duet_vectors_(novagen)&plasmid=pET-9dס The Sybodie: https://www.biorxiv.org/content/10.1101/2020.04.16.045419v1.abstract

References